腺相关病毒属微小病毒( parvovirus)家族的成员，为无包膜的单链线状DNA病毒。AAV的基因组约4700bp。基因组5‘、3’端各有一个145nt的反向末端重复序列（ITR）。在两个ITR之间存在两个开放阅读框，其中Rep基因参与病毒的复制和整合，Cap基因编码病毒衣壳蛋白。

                                                       图1 AAV病毒基因组

腺相关病毒具有安全性高、免疫原性低、能介导基因在动物体内长期稳定表达，因而被认为是最有潜力成为基因治疗工具的载体而在国内外研究中广泛应用。

应用优势：动物和临床试验
AAV进入细胞前，首先要通过衣壳蛋白与细胞表面的相应受体结合，进而附着于细胞表面，随后通过衣壳蛋白与其它复合受体的相互作用来起始病毒颗粒的细胞内转运等生物学活动。AAV不同血清型有不同的衣壳蛋白，进而可与不同的细胞表面受体结合，体现出血清型对不同组织器官的不同亲和性。百恩维生物在包装腺相关病毒时有9种不同的AAV血清型可供客户选择，建议客户针对不同组织器官选择相应血清型的AAV病毒，见下表。

服务流程：

       您只需给我们提供详细的基因信息，选定所需的腺相关病毒载体，我们就能为您生产出符合您要求的带有目的基因的腺相关病毒。
       另外百恩维生物经过鉴定与质量控制：载体测序鉴定、Q-PCR法测定滴度、感染活性测定等，保证给您提供的病毒正确可靠。

Biowit腺相关病毒包装技术路线：

• 腺相关病毒操作手册查看>>

1.Overexpression of 1 ankyrin repeat domain 1 enhances cardiomyocyte apoptosis by promoting p53 activation and mitochondrial dysfunction in rodents
Liang SHEN*#, Ci CHEN*#, Xuan WEI*, Xixian LI*, Guangjin LUO*, Jingwen ZHANG *, Jianping BIN*, Xiaobo HUANG*, Shiping CAO*, Guofeng LI†, Yulin LIAO*
*State Key Laboratory of Organ Failure Research,Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
†Department of Pharmacy, Nanfang Hospital, Southern Medical University,Guangzhou 510515, China
# These authors contributed equally to this work.
【pAAV2/9-CMV-ZsGreen (AAV: adeno-associate virus) 34 vectors carrying sh-Ankrd1 (short hairpin of Ankrd1) or negative control were generated by a 35 professional company (Biowit, Shenzheng, China). 】
 

2.Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo
SHAN REN, YONGJUN LIU, JINFENG MA, YONG LIU, ZEZHENG DIAO, DELING YANG, XU ZHANG, YONGMING XI and YOUGU HU
Department of Spine Surgery, Affiliated Hospital of Medical College,Qingdao University, Qingdao, Shandong 266003, P.R. China
【Recombinant AAV (rAAV)-OP1, rAAV-SOX9 and rAAV-EGFP were provided by Biowit Technologies Co., Ltd. (Shenzhen, China). 】
 

3. VEGF-Mediated Proliferation of Human Adipose Tissue-Derived Stem Cells
Guangfeng Chen1., Xiujuan Shi1., Chen Sun1, Min Li2, Qing Zhou2, Chen Zhang1, Jun Huang1, Yu Qiu1,
Xiangyi Wen1, Yan Zhang1, Yushan Zhang1, Shuzhang Yang1, Lixia Lu1, Jieping Zhang1, Qionglan Yuan1,
Jianwei Lu1, Guotong Xu1, Yunyun Xue1, Zibing Jin3, Cizhong Jiang2*, Ming Ying1,4*, Xiaoqing Liu1,4*
 （1 Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China, 2 Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China, 3 Division of Ophthalmic Genetics, The Eye Hospital of Wenzhou Medical College and Lab for Stem Cell & Retinal Regeneration, School of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou, China, 4 Shenzhen Key Laboratory of Marine Bioresources and Ecology,College of Life Sciences, Shenzhen University, Shenzhen, China）
【Short hairpin RNA (shRNA) for the VEGF gene silencing in an adeno-associated viral vector (AAV) were constructed and packaged into viral particles with a titer of over 161012/ml by BioWit Technologies Ltd. Lentiviral vector harboring microRNAs were constructed into pLVX-ShRNA2 vector and particles were produced by using 2-generation packaging mix (BioWit Technologies Ltd.) with over a titer of over 16108 TU.】
【A lentiviral vector with a putative microRNA 21-recognition element (single copy) from the VEGF gene fused with the firefly luciferase gene as a 39-untranslated region was provided by BioWit Technologies Ltd.】
【The cell pellet was resuspended in DMEM/F12 medium (GIBCO) supplemented with 10% FBS (GIBCO), 10%KSR (Invitrogen) or ADSC serum-free media (Biowit Technologies Ltd, Quincy, USA) at a density of 26106 cell/mL.】
 

4. Cytosolic CARP Promotes Angiotensin II- or PressureOverload-Induced Cardiomyocyte Hypertrophy through
Calcineurin Accumulation
Ci Chen1, Liang Shen1, Shiping Cao1*, Xixian Li1, Wanling Xuan1, Jingwen Zhang1, Xiaobo Huang1,Jianping Bin1, Dingli Xu1, Guofeng Li2, Masafumi Kitakaze1,3, Yulin Liao1*
1 State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China,
2 Department ofPharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, China,
3 Cardiovascular Division of the Department of Medicine, National Cerebral and Cardiovascular Center, Osaka, Japan
【pAAV2/9-CMV-ZsGreen vectors carrying short hairpin RNA targetingAnkrd1 (sh-Ankrd1) or scramble were created by a professional company (Biowit, Shenzheng, China).】
 

5. Regulation of RyR2 Channels of Mongrel canines with Heart Failure by Means of FKBP12.6 Gene
Juan Sun, Yanmei Lu, Yan Huang, Ling Zhang, Yitong Ma
Ma/Exp Clin Cardiol Vol 20 Issue8 pages 2792-2811 / 2014
【AAV9/EGFP and AAV9/FKBP12.6-EGFP were purchased from Shenzheng BioWit Technologies Co., Ltd and its titer were1.0×1012vg/ml.】
 

6. miR-29a-3p Attenuates Hypoxic Pulmonary Hypertension by Inhibiting Pulmonary Adventitial Fibroblast Activation
Ying Luo, Hai-Ying Dong, Bo Zhang, Zhao Feng, Yi Liu, Yu-Qi Gao, Ming-Qing Dong and Zhi-Chao Li
Hypertension. published online November 24, 2014;
Hypertension is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Copyright © 2014 American Heart Association, Inc. All rights reserved.
Print ISSN: 0194-911X. Online ISSN: 1524-4563
【annealed and cloned into pAAV-ZsGreen-miRNA vector (Biowit Technologies, Shenzhen, China) to construct the target vector (refereed as AAV5-miR-29a) according to the manufacturer’s instruction.】
 

7. Galectin-9 ameliorates respiratory syncytial virus-induced pulmonaryimmunopathology through regulating the balance between Th17 andregulatory T cells
Xiaoxia Lua,∗,1, Karen S. McCoyb,1, Jiali Xua, Weikun Huc, Hebin Chena, Kun Jianga,Feng Hana, Peng Chena, Yanli Wanga
a.Department of Pulmonary Medicine, Wuhan Children’s Hospital, Wuhan, China
b.Department of Pediatrics, Division of Pulmonary Medicine, Nationwide Children’s Hospital and Ohio State University School of Medicine and PublicHealth, Columbus, OH, USA
c.Department of Ophthalmology, Tongi Hospital, Tongi Medical College, Huazhong University of Science and Technology, Wuhan, China
【The rAAV9-Gal-9 and rAAV9-ZsGreen viral particles were gen-erated using the HET kit following the manufacturer’s instructions(Biowit Technologies, China). 】
 

8. miR-134 Regulates Ischemia/Reperfusion Injury-Induced Neuronal Cell Death by Regulating CREB Signaling
Weidong Huang & Xiaobin Liu & Jie Cao & Facai Meng &Min Li & Bo Chen & Jie Zhang
【Recombinant adeno-associated virus (AAV) vectors were constructed by Biowit Technologies (Biowit, Shenzhen,China).】
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9. pAAV-hSOX9-IRES-tdTomato recombinant plasmid constructs adeno-related virus package
Diao Ze-zheng, Yan Guo-qing, Zhang Zhi-wei, Fang Jing, Xu Peng, Xi Yong-ming, Ren Shan, Liu Yong-jun, Sui Ai-hua
Department of Spinal Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
 

10. VEGF165基因重组腺相关病毒构建及转染猪骨髓间充质干细胞的研究
 夏利龙； 朱成楚； 陈保富； 张波； 陈宇； 胡炳川； 张健；
温州医科大学附属台州医院；
【1．主要试剂：细胞AAV 包装质粒pAAV-IRES-Zs-Green、JM109感受态细菌、HET转染试剂盒(深圳百恩维生物科技有限公司)】
 

11. 腺相关病毒载体介导ZsGreen 基因通过3 种转染途径在心肌组织转染效果的比较
谭文鹏，杨侃，李安莹，杨琼
（中南大学湘雅三医院心内科，长沙410013）
【携带报告基因ZsGreen 的腺相关病毒-9（Adeno-associated virus 9-ZsGreen，AAV9-ZsGreen）购自百恩维生物公司】
 

12. 同一滴度不同方法转染AAV9-SERCA2a 基因对大鼠的有效性和毒副作用评价
徐晓霞1，周祁娜1，姜涛2，王红丽2，王欢1，季萌1，王凌鹏1，张广伟1，侯月梅1
( 1. 新疆医科大学第一附属医院心律失常科，乌鲁木齐830054;2.新疆医科大学第一附属医院医学研究中心，乌鲁木齐830054)
【携带EGFP 的9 型腺相关病毒( AAV9-EGFP) 、携带SERCA2a-EGPF 的9 型腺相关病毒( AAV9-
SERCA2a-EGFP) 购自深圳市百恩维生物科技有限公司】
 

13. 9型重组腺相关病毒转染microRNA-21 前体在大鼠心肌的转染效率及安全性
杨琼1，杨侃1，李安莹1，谭文鹏2
(1.中南大学湘雅三医院心内科，湖南长沙410013；2.广州医学院第三附属医院心内科，广东广州510150）【rAAV9- Zs-Green-pre-miR-21 由深圳百恩维生物公司构建】
 

腺相关病毒可用于基因的过表达（3kb以下）、shRNA（单条或者多条串联）、miRNA（pre-miRNA或者pri-miRNA）、可诱导表达。腺相关病毒载体有CMV、mCMV、CAG、PGK、UbC、EF1a、GFAP等多种启动子可供选择，常用载体如下表。

               肝脏（整体）                         心脏（横切）                             脑部

             视网膜                                    视神经                              视网膜神经节